A Quantitative Fractionation Method for Bilirubin in Human Bile
نویسندگان
چکیده
منابع مشابه
The isolation and characterization of bilirubin diglucuronide, the major bilirubin conjugate in dog and human bile.
The chemical structure of the major conjugate of bilirubin was unequivocally elucidated by structural analysis. The conjugated bilirubins were first separated from the lipid components of human duodenal aspirates or dog gall-bladder bile, and then resolved by t.l.c. into a series of tetrapyrroles. The major tetrapyrrole was then converted into its more stable dipyrrolic azo derivative for furth...
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1. Conjugated bile pigments, separated in two fractions by semi-quantitative t.l.c. performed on silicic acid with phenol/water as the developing solvent, were treated with diazotized ethyl anthranilate. Resulting dipyrrylazo derivatives were analysed by quantitative t.l.c. 2. The tentative structure elucidation of tetrapyrrolic bilirubin conjugates and semi-quantitative evaluation of rat bile,...
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Interactions of unconjugated bilirubin with bile salts.
The rate of peroxidation of unbound, unconjugated bilirubin (UCB) was used to assess the interactions of UCB with four taurine-conjugated bile salts at pH 8.2, 37 degrees C, and an ionic strength of 0.15. Each of the four structurally different bile salts markedly decreased the rate of peroxidation of UCB in the presence of horseradish peroxidase (HRP); 30% of UCB was bound even at low, premice...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1965
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.84.329